Objective To explore the expression of tripartite motif-containing protein 44 TRIM44 in laryngeal squamous cell carcinoma LSCC and its correlation with prognosis. Method A total of 98 patients with LSCC admitted to Zigong First People's Hospital from October 2018 to October 2019 were selected and their LSCC tissues and paracancer tissues were respectively taken during operation. The expression level of TRIM44 mRNA in LSCC tissues and adjacent tissues was detected by qRT-PCR. According to the mean value of TRIM44 mRNA expression in LSCC tissues they were divided into low TRIM44 expression group and high TRIM44 expression group. The relationship between TRIM44 mRNA expression level and survival status of LSCC patients was analyzed. To analyze the risk factors affecting the poor prognosis of LSCC patients. Result The positive expression rate of TRIM44 in LSCC tissues was significantly higher than that in paracancer group P < 0. 05 . The expression level of TRIM44 mRNA in LSCC tissues was significantly increased compared with that in adjacent tissues P<0. 05 . TRIM44 mRNA expression level was correlated with lymph node metastasis differentiation degree and distant metastasis P < 0. 05 . Analysis showed that the 3-year survival rate of patients with high expression of TRIM44mRNA was lower than that of patients with low expression of TRIM44 P<0. 05 . There were significant differences in the proportion of poorly differentiated lymph node metastasis and distant metastasis between surviving patients and deceased patients P < 0. 05 . Lymph node metastasis HR=3. 332 and high expression of TRIM44 mRNA HR= 5. 365 were risk factors for poor prognosis in LSCC patients P<0. 05 . Conclusion TRIM44 mRNA is highly expressed in LSCC tissues which is related to the clinical characteristics and survival of patients with LSCC and is expected to be a potential prognostic biomarker for LSCC.