目的 通过基因表达汇编公共数据库，探讨 Xklp2 靶蛋白在膀胱癌中的表达情况，并进一步探索其与膀胱癌临床 病理特征的关系，评价TPX2 对膀胱癌术后患者预后评价的意义，预测TPX2 推动膀胱癌发生和发展机制。方法 从 NCBI 的基因表达汇编数据库收集膀胱肿瘤相关的公共数据集，对其中的表达谱资料及相关临床资料进行分析；基于GSEA3.0， 使用基因富集分析方法，分析TPX2 调控的基因通路。结果 TPX2 在膀胱癌组织中为高表达（P ＜ 0.0001）。不同年龄 （P=0.027）、性别（P=0.040）、T 分期（P=0.001）、N 分期（P=0.013）、进程（progression，P=0.002）和分级水 平（grade）中，TPX2 的表达存在明显差异。TPX2 的表达水平与膀胱癌患者预后总生存率和是否复发相关（P ＜ 0.05）； TPX2 表达水平高的样本富集了与精子发生、未折叠蛋白反应、PI3K/AKT/mTOR 通路、mtorc1 信号通路、胆固醇平衡、有丝分裂、糖酵解、G2M 检查点、E2F 转录因子、癌基因 myc 有关的基因集。结论 TPX2 在膀胱癌中为高表达，与膀胱癌多 个病理性指标相关，且可以作为潜在的判断膀胱癌患者预后的标志物和治疗肿瘤的靶标。

Objective To clarify the connection between TPX2 expression and clinicopathological characteristics of bladder cancer, so as to evaluate the function of TPX2 as a prognosis marker in bladder cancer. Methods GEO datasets were collected and expression profile and clinical information were analyzed. GSEA was conducted to explore the gene sets enriched in TPX2 high-expression samples. Results The expression of TPX2 was up-regulated in bladder cancer (P<0.0001); TPX2 expression was significantly associated with age, sex, T stage, N stage, progression, grade. Higher expression of TPX2 indicated poor prognosis in bladder cancer. GSEA indicated that TPX2 regulates gene sets associated with spermatogenesis,unfolded protein response, PI3K/ AKT/mTOR signaling,mTORC1 signaling, cholesterol homeostasis, mitotic spindle, glycolysis, G2M checkpoint, E2Ftargets, myctargets. Conclusions TPX2 is highly expressed in multiple tumors and functions as potential marker and target in diagnosis and treatment in bladder cancer.