Objective To clarify the connection between TPX2 expression and clinicopathological characteristics of bladder cancer, so as to evaluate the function of TPX2 as a prognosis marker in bladder cancer. Methods GEO datasets were collected and expression profile and clinical information were analyzed. GSEA was conducted to explore the gene sets enriched in TPX2 high-expression samples. Results The expression of TPX2 was up-regulated in bladder cancer (P<0.0001); TPX2 expression was significantly associated with age, sex, T stage, N stage, progression, grade. Higher expression of TPX2 indicated poor prognosis in bladder cancer. GSEA indicated that TPX2 regulates gene sets associated with spermatogenesis,unfolded protein response, PI3K/ AKT/mTOR signaling,mTORC1 signaling, cholesterol homeostasis, mitotic spindle, glycolysis, G2M checkpoint, E2Ftargets, myctargets. Conclusions TPX2 is highly expressed in multiple tumors and functions as potential marker and target in diagnosis and treatment in bladder cancer.