Abstract: Objective Renal cell carcinoma is induced by a variety of factors. The expression of miR-205 in renal cell carcinoma and renal cell carcinoma was studied. The relationship between the clinicopathological characteristics and prognosis of patients with renal cell carcinoma and the expression of miR-205, and the mechanism of miR-205 were elucidated. Methods From February 2010 to February 2014, 60 cases of renal carcinoma tissue and normal tissue samples were selected from the Department of Urology at our hospital. Expression level of miR-205 was detected by RT-PCR analysis. We observed the function of miR-205 on biological behavior of ACHN cells in vitro, the ACHN cell was transfected with miR-205 to up-regulate miR-205 expression. MTT was used for evaluating cell proliferation in miR-205 up-expression. Transwell assays were employed for investigating the cell invasion and migration ability inmiR-205up-expression. Immunohistochemical was used to investigate the Epithelial mesenchymal transformation in ACHN cell. Results The expression of miR-205 was detected in renal carcinoma tissues and normal renal tissues, but the renal carcinoma tissues showed a significantly lower expression. The expression of miR-205 was correlated with infiltration and relapse of renal carcinoma (P<0.05), but showed no correlation with pathological stage or clinical stage of renal carcinoma (P<0.05). After mir-205 overexpression, it was found that the growth of renal cancer cells was significantly inhibited (P<0.05), and the migration efficiency was significantly reduced (P<0.05). There is epithelial mesenchymal transformation in renal carcinoma cells, and mir205 may inhibit cell proliferation and migration by inhibiting epithelial mesenchymal transformation. Conclusions The decreased expression of miR-205 were correlated with the progress of renal carcinoma. This result may provide new ideas for studying the molecular mechanism of renal carcinoma and develop a new therapeutic strategy for renal carcinoma in the future.