Ampelopsin induces cell growth inhibition in breast cancer cells by suppressing pyruvate kinase M2-dependent glycolysis

ZHOU Yong; ZHENG Jin-ying; XU Xiao-hua; JIN Xiao-ling

doi:10.16689/j.cnki.cn11-9349/r.2019.02.016

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Electronic Journal of Metabolism and Nutrition of Cancer ›› 2019, Vol. 6 ›› Issue (2) : 236-241. DOI: 10.16689/j.cnki.cn11-9349/r.2019.02.016

Ampelopsin induces cell growth inhibition in breast cancer cells by suppressing pyruvate kinase M2-dependent glycolysis

ZHOU Yong， ZHENG Jin-ying， XU Xiao-hua， JIN Xiao-ling

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Abstract

Objective To explore whether AMP， a flavonol mainly found in Ampelopsis grossedentata， induces cell growth inhibition by suppressing glycolysis through Akt-mTOR/PKM2 pathway in breast cancer cells. Methods After MCF-7 and MDA- MB-231 breast cancer cells treated with 20， 40， 60 and 80μmol/L AMP for 24h， cell viability was assessed by CCK-8 assay， glucose uptake assay was performed using 2-NBDG， lactate levels were measured using the colorimetric lactate assay kit and the protein expressions were assessed by Western blot. Results AMP treatment could inhibit cell viability in MCF-7 and MDA- MB-231 breast cancer cells in dose-dependent manners. The further experiments exhibited that AMP successfully blocked cell glycolysis by inhibiting the level of glucose uptake and the production of lactic acid， decreased the level of glycolysis-related proteins pyruvate kinase M2 (PKM2)， glucose transporter1 (GLUT1) and lactate dehydrogenase A (LDHA)， and also inactivated the Akt-mTOR pathway by down-regulation of p-Akt、p-mTOR、p-p70S6K expressions. However， Akt-mTOR pathway inducer IGF-1 treatment significantly attenuated AMP- induced above mentioned results. Conclusion AMP suppressed breast cancer cell growth and glycolysis through inhibiting Akt-mTOR/PKM2 pathway， and may provide a potential therapeutic target for breast cancer treatment.

Key words

Ampelopsin / Breast cancer cells / Glycolysis / PKM2 / Akt-mTOR pathway

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ZHOU Yong， ZHENG Jin-ying， XU Xiao-hua， JIN Xiao-ling. Ampelopsin induces cell growth inhibition in breast cancer cells by suppressing pyruvate kinase M2-dependent glycolysis[J]. Electronic Journal of Metabolism and Nutrition of Cancer. 2019, 6(2): 236-241 https://doi.org/10.16689/j.cnki.cn11-9349/r.2019.02.016

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