L-carnitine improves skeletal muscle cell atrophy of cancer cachexia by regulating lipid metabolism

1; 2ZHU Ming-xing; 1WU Chang-peng; 3YIN Liang-yu; 1LU Zong-liang; 1LI Na; 1LIU Jie; 1GUO Jing; 1XU Hong-xia

doi:10.16689/j.cnki.cn11-9349/r.2019.03.010

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Electronic Journal of Metabolism and Nutrition of Cancer ›› 2019, Vol. 6 ›› Issue (3) : 320-325. DOI: 10.16689/j.cnki.cn11-9349/r.2019.03.010

L-carnitine improves skeletal muscle cell atrophy of cancer cachexia by regulating lipid metabolism

1，2ZHU Ming-xing， 1WU Chang-peng， 3YIN Liang-yu，1LU Zong-liang， 1LI Na，1LIU Jie， 1GUO Jing， 1XU Hong-xia

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Abstract

Objective To explore the underlying mechanisms of L-carnitine on improving cancer cachexia. Methods 100ng/ml TNF-α was used in murine myoblast cell-line C2C12 to induce cell atrophy. Cell model of cancer cachexia associated skeletal muscle loss was established. Differentiation status of skeletal muscle fiber treated by different doses of L-carnitine was observed under microscope after stained by crystal violet. Lipid metabolism related protein expressions in C2C12 were analyzed using Western Blot to explore the underlying mechanisms of L-carnitine on improving cancer cachexia. Results 100μg/ml and 1000μg/ml L-carnitine treatments induced differentiation of C2C12 and increased the diameter of skeletal muscle fiber (P<0.001). TNF-α group compared with the control group， the expression of CPT-I and PGC-1α decreased， while FOXO1， CD36 and PDK4 elevated. L-carnitine induced PGC1-α and CPT-I expressions but inhibited FOXO1 and CD36 expressions. L-carnitine had no effects on PDK4 expression. Conclusion L-carnitine improves cancer associated cachexia and increases skeletal muscle protein via regulating lipid metabolism capacity.

Key words

L-carnitine / TNF-α / C2C12 / Cancer cachexia / Lipid metabolism

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1，2ZHU Ming-xing， 1WU Chang-peng， 3YIN Liang-yu，1LU Zong-liang， 1LI Na，1LIU Jie， 1GUO Jing， 1XU Hong-xia. L-carnitine improves skeletal muscle cell atrophy of cancer cachexia by regulating lipid metabolism[J]. Electronic Journal of Metabolism and Nutrition of Cancer. 2019, 6(3): 320-325 https://doi.org/10.16689/j.cnki.cn11-9349/r.2019.03.010

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