Mechanism of miR-125b inhibiting proliferation of glioma cells

1Chen Pengfei; 1Huo Xianhao; 1Liu Wenqing; 1Li Kai; 2Xue Tao

doi:10.16689/j.cnki.cn11-9349/r.2020.01.012

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Electronic Journal of Metabolism and Nutrition of Cancer ›› 2020, Vol. 7 ›› Issue (1) : 64-69. DOI: 10.16689/j.cnki.cn11-9349/r.2020.01.012

Mechanism of miR-125b inhibiting proliferation of glioma cells

1Chen Pengfei,1Huo Xianhao,1Liu Wenqing,1Li Kai, 2Xue Tao

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Abstract

Objective To explore the molecular mechanism of miR-125b inhibiting proliferation of glioma cells. Methods A total of 32 glioma patients were included in the research from December 2016 to December 2017, 31 patients who were with cerebral hemorrhage undergoing intracerebral hematoma removal or with intracranial decompression due to craniocerebral injury but normal brain tissuenormal were collected as the control. The expressions of microRNAs-125b in glioma tissue and LN229 cells were detected by qPCR. The inhibitory effect of microRNAs-125b on the growth of LN229 cells was detected after transfecting microRNAs-125b mimics. The target of microRNAs-125b was predicted by targetscan, miRanda and other prediction softwares. Dual luciferase reporter gene system was used to detect the interaction between RNA-125b and STAT3 mRNA, and the effect of RNA-125b on the expression of STAT3 protein in LN229 cells was detected by Western blot. Results Compared with normal para-cancerous brain tissues, the expression of microRNA-125b in glioma tissue was significantly decreased, and the expression of microRNA-125b in glioma cell line LN229 was also significantly lower than that in normal astrocytes. Compared with NC mimics, the proliferation of LN229 cells was significantly inhibited after transfected microRNA-125b mimics. Dual luciferase reporter gene system studies have shown that the transcriptional activity of STAT3 can be directly regulated by microRNAs-125b which also significantly inhibit the expression of STAT3 protein in LN229 cells. Conclusion The low expression of miR-125b in glioma cells is associated with poor prognosis. MiR-125b can regulate the proliferation of glioma cells by inhibiting the expression of STAT3.

Key words

Glioma / Proliferation / Signal transducer and activator of transcription / Mechanism

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1Chen Pengfei,1Huo Xianhao,1Liu Wenqing,1Li Kai, 2Xue Tao. Mechanism of miR-125b inhibiting proliferation of glioma cells[J]. Electronic Journal of Metabolism and Nutrition of Cancer. 2020, 7(1): 64-69 https://doi.org/10.16689/j.cnki.cn11-9349/r.2020.01.012

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