Study on the mechanism of miR-4298 targeting TGIF2 influencing the proliferation and migration of multiple myeloma cells

Du Jingzhu; Chen Qi; Yin Hao; Li Feng

doi:10.16689/j.cnki.cn11-9349/r.2020.02.017

PDF(2408 KB)

Electronic Journal of Metabolism and Nutrition of Cancer ›› 2020, Vol. 7 ›› Issue (2) : 225-230. DOI: 10.16689/j.cnki.cn11-9349/r.2020.02.017

Study on the mechanism of miR-4298 targeting TGIF2 influencing the proliferation and migration of multiple myeloma cells

Du Jingzhu， Chen Qi， Yin Hao， Li Feng

Author information +

History +

Abstract

Objective To investigate the effect of microRNA-4298 （miR-4298） on proliferation， migration and invasion of multiple myeloma cells and its possible mechanism. Methods Fluorescence quantitative PCR （qPCR） was used to detect the expression level of miR-4298 in multiple myeloma cell lines （U-266， SKO-007， RPMI-8226）； RPMI-8226 cells were randomly divided into the control group （conventional cultured cells）， the miR -NC group （negative control of miR-4298 transfection）， and the miR-4298 group （miR-4298 mimic transfection）； qPCR detection of transfection effect， using cell count （CCK-8）， cell scratch and Transwell test respectively RPMI-8226 cell proliferation， migration and invasion ability， TargetScan prediction， dual luciferase reporter gene verification miR-4298 and transforming growth interaction factor 2 （IGFIF2） targeting relationship； Western blot detection of TGIF2 protein levels.Results The expression of miR-4298 in multiple myeloma cell lines （U-266， SKO-007， RPMI-8226） were down-regulated （P<0.05）. Up-regulation of miR-4298 inhibited the proliferation of RPMI-8226 cells （P<0.05）， and decreased the migration and invasion of RPMI-8226 cells （P<0.05）. TGIF2 was the downstream target gene of miR-4298. miR-4298 was negatively correlated with TGIF2 protein level. Up-regulation of TGIF2 could reverse the inhibitory effect of miR-4298 on proliferation， migration and invasion of RPMI-8226 cells. Conclusion miR-4298 is down-regulated in multiple myeloma cell lines， which may inhibit the proliferation， migration and invasion of RPMI-8226 cells by inhibiting TGIF2.

Key words

Multiple myeloma / miR-4298 / Transforming growth interaction factor 2

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

Du Jingzhu， Chen Qi， Yin Hao， Li Feng. Study on the mechanism of miR-4298 targeting TGIF2 influencing the proliferation and migration of multiple myeloma cells[J]. Electronic Journal of Metabolism and Nutrition of Cancer. 2020, 7(2): 225-230 https://doi.org/10.16689/j.cnki.cn11-9349/r.2020.02.017

References

1.MICHELS T C， PETERSEN K E. Multiple myeloma: diagnosis and treatment［J］. Am Fam Physician， 2017， 95（6）:373-383.
2.LI G， LUO J， QIU X， et al. Prediction of microRNA-disease associations with a Kronecker kernel matrix dimension reduction model［J］. Rsc Advances， 2018， 8（8）:4377-4385.
3.LIU X， HAN Z， YANG C . Associations of microRNA single nucleotide polymorphisms and disease risk and pathophysiology［J］. Clin Genet， 2016， 92（3）:235-242.
4.徐艳妮，肖翠容，黄英丹，等. 循环血清中miRNA作为多发性骨髓瘤诊断标志的研究［J］. 中国实验血液学杂志， 2017， 25（2）:471-475.
5.RAHAT M A， PREIS M. Role of microRNA in regulation of myeloma-related angiogenesis and survival［J］. World J Hematol， 2016， 5（2）: 51-60.
6.BONG I P N， NG C C， BAHARUDDIN P， et al. MicroRNA expression patterns and target prediction in multiple myeloma development and malignancy［J］. GENES GENOM， 2017， 39（5）: 533-540.
7.陈文明. 复发难治性多发性骨髓瘤的治疗:《中国多发性骨髓瘤诊治指南（2015年修订）》解读［J］. 中华内科杂志， 2016， 55（2）:93-94.
8.VOS PD， LEEDMAN PJ， FILIPOVSKA A， et al. Modulation of miRNA function by natural and synthetic RNA-binding proteins in cancer［J］. Cell Mol Life Sci. 2019；76（19）:3745-3752.
9.MARKOPOULOS G S， ROUPAKIA E， TOKAMANI M， et al. A step-by-step microRNA guide to cancer development and metastasis［J］. Cell Oncol， 2017， 40（4）:303-339.
10.GAO D， XIAO Z， LI H P， et al. The mechanism study of miR-125b in occurrence and progression of multiple myeloma［J］. Cancer Med， 2018， 7（1）:134-145.
11.YU T， ZHANG X， ZHANG L， et al. MicroRNA-497 suppresses cell proliferation and induces apoptosis through targeting PBX3 in human multiple myeloma［J］. Am J Cancer Res， 2016， 6（12）:2880-2889.
12.UMEZU T， TADOKORO H， AZUMA K， et al. Exosomal miR-135b shed from hypoxic multiple myeloma cells enhances angiogenesis by targeting factor-inhibiting HIF-1［J］. Blood， 2014， 124（25）:3748-3757.
13.YANG Y， LI F， SAHA M N， et al. miR-137 and miR-197 Induce Apoptosis and Suppress Tumorigenicity by Targeting MCL-1 in Multiple Myeloma［J］. Clin Cancer Res， 2015， 21（10）:2399-2411.
14.丰茂晓，古春明，阴钊，等. miR-19a和miR-92a在多发性骨髓瘤中的功能及其信号通路分析［J］. 中国病理生理杂志， 2015， 31（8）:1505-1509.
15.LEONE E， MORELLI E， MARTINO M T D， et al. Targeting miR-21 inhibits in vitro and in vivo multiple myeloma cell growth［J］. Clin Cancer Res， 2013， 19（8）:2096-2106.
16.GULYAEVA L F， KUSHLINSKIY N E. Regulatory mechanisms of microRNA expression［J］. J Transl Med， 2016， 14（1）:143-156.
17.杨亚丽，宋超，符辉. TGIF2调控胶质瘤细胞的增殖和迁移［J］. 中国组织化学与细胞化学杂志， 2017， 26（1）:1-6.
18.WU S， HE X， LI M， et al. MiRNA-34a overexpression inhibits multiple myeloma cancer stem cell growth in mice by suppressing TGIF2［J］. Am J Transl Res， 2016， 8（12）:5433-5443.
19.LU Y J， LIU R Y， HU K， et al. MiR-541-3p reverses cancer progression by directly targeting TGIF2 in non-small cell lung cancer［J］. Tumour Biol， 2016， 37（9）:12685-12695.
20.YANG H， QINGHA P， BIN C， et al. MicroRNA-34a inhibits tumor invasion and metastasis in gastric cancer by targeting Tgif2［J］. Int J Clin Exp Pathol，2015， 8（8）:8921-8928.