Effects of docosahexaenoic acid combined with cisplatin on proliferation and apoptosis of gastric cancer cell MGC-803

Xu Liben; Wu Chaoyang; Wang Yuandong

doi:10.16689/j.cnki.cn11-9349/r.2020.03.013

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Electronic Journal of Metabolism and Nutrition of Cancer ›› 2020, Vol. 7 ›› Issue (3) : 317-320. DOI: 10.16689/j.cnki.cn11-9349/r.2020.03.013

Effects of docosahexaenoic acid combined with cisplatin on proliferation and apoptosis of gastric cancer cell MGC-803

Xu Liben, Wu Chaoyang, Wang Yuandong

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Abstract

Objective Study the effects of docosahexaenoic acid combined with cisplatin on the proliferation and apoptosis of human gastric cancer MGC-803 cells, to analyze its possible mechanisms. Methods MTT evaluate the repression effect of DHA combined with DDP on MGC803 cells. calculate the IC50 and Q values. Observed apoptosis and morphology by DAPI, The cell cycle distribution and apoptosis rate were examined by flow cytometry.The expressions of survivin/NF-κB proteins were detected by immunohistochemical method. Results The proliferation of MGC803 cells was inhibited by DHA in a time-dose dependent manner. The inhibitory effect of combined group on the proliferation of MGC-803 cells were more obvious. Q>0.85, The DHA strengthen the cytotoxic effect of DDP on MGC803 cells. Drug treatment showed characteristics of apoptosis by using fluorescence microscopy.The apoptotic rate of DHA group, the apoptotic rate of combination group was higher than single drug group (P<0.05). The number of the combination group cells in G0/G1 phase increased (P<0.01), the number of G2/M and S phase cells decreased (P=0.01). The expression levels of survivin and NF-κB protein in combined group were lower than single drug group (P<0.05). Conclusion DHA can synergistic inhibit the proliferation of MGC803 cells, related to the regulation of cell cycle, down regulation of survivin and NF-κB protein expression, induction of apoptosis and enhance-ment of chemosensitivity.

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Xu Liben, Wu Chaoyang, Wang Yuandong. Effects of docosahexaenoic acid combined with cisplatin on proliferation and apoptosis of gastric cancer cell MGC-803[J]. Electronic Journal of Metabolism and Nutrition of Cancer. 2020, 7(3): 317-320 https://doi.org/10.16689/j.cnki.cn11-9349/r.2020.03.013

References

1.JING K, WU T, LIM K. Omega-3 polyunsaturated fatty acid and cancer ［J］. Anticancer Agents Med Chem, 2013, 13(8):1162-1177.
2.PETTERSEN K, MONSEN V T, HAKVAG PETTERSEN C H, et al. DHA-induced stress response in human colon cancer cells focus on oxidative stress and autophagy［J］. Free Radical Bio Med, 2016, 90:158-167.
3.YIN Y, SUI C, MENG F, et al. The omega-3 polyunsaturated fatty acid docosahexaenoic acid inhibits proliferation and progression of non-small cell lung cancer cells through the reactive oxygen species-mediated inactivation of the PI3K/Akt pathway ［J］. Lipids Health Dis, 2017, 16(1):87-95.
4.TANAKA H, KAGEYAMA K, KIMURA M, et al. Promotive effects of hyperthermia on the inhibition of DNA synthesis in ehrlich ascites tumor cells by eicosapentaenoic and docosahexaenoic acids ［J］. Exp Oncol, 2006, 28(3):203-208.
5.KATO T, KOLENIC N, PARDINI R S. Docosahexaenoic acid (DHA), a primary tumor suppressive omega-3 fatty acid, inhibits growth of colorectal cancer independent of p53 mutational status ［J］. Nutr Cancer, 2007, 58(2):178-187.
6.MOCELLIN M C, PASTORES J A, CAMARGO C Q, et al. Fish oil decreases C-reactive protein/albumin ratio improving nutritional prognosis and plasma fatty acid profile in colorectal cancer patients ［J］. Lipids, 2013, 48(9):879-888．
7.CHA M C L, MECKLING K A. Low dose docosahexaenoic acid protects normal colonic epithelial cells from araC toxicity ［J］. BMC Pharmacol, 2005, 5(1):5-7.
8.MENENDEZ J A, LUPU, COLOMER R. Exogenous supplementation with omega-3 polyunsaturated fatty acid docosahexaenoic acid (DHA) synergistically enhances taxane cytotoxicity and downregulates Her-2/neu oncogene expression in human breast cancer cells ［J］.Eur J Cancer Prev, 2005, 14(3):263-270.
9.RAKATZI I, MUELLER H, RITZELER O, et al. Adiponectin counteracts cytokine and fatty acid induced apoptosis in the pancreatic betacell line INS-1［J］. Diabetologia, 2004, 47(2):249-258.
10.尹勇, 彭俊生, 詹文华. ω-3多不饱和脂肪酸对人胃癌细胞系生长的抑制作用及其机制［J］. 中华胃肠外科杂志, 2004, 7(3):233-237.
11.楼健颖, 吴丹, 林汉庭,等. ω-3多不饱和脂肪酸二十二碳六烯酸对胃癌细胞株AGS生长和增殖的影响［J］. 中华普通外科杂志, 2007, 22(8):613-615.
12.CHOU T C. Preclinical versus clinical drug combination studies ［J］. Leuk Lymphoma, 2008, 49(11):2059-2080．
13.杨平, 陈博, 伍小汀. 胃癌住院患者营养风险及临床营养支持现状调查［J］. 中国普外基础与临床杂志, 2011, 18(6):620-623．
14.江志伟, 黎介寿. 瘤营养学的指南与实践［J］. 肠外与肠内营养, 2012, 19(1):1-2．
15.LEE S E, LIM J W, KIM H. Activator protein-1mediates docosahexaenoic acid induced apoptosis of human gastric cancer cells［J］. Ann NY Acad Sci, 2009, 1171:163-169.
16.SHENG H, LI P, CHEN, et al. Omega-3 PUFAs induce apoptosis of gastric cancer cells via ADORAI［J］.Front Bio sci, 2014, 19:854-861.
17.司徒镇强, 吴军正. 细胞培养［M］. 西安: 世界图书出版公司, 2004.
18.王柯柯, 蒋亦燕, 洪丹,等.DHA联合5-FU通过Rho/ROCK调控胃癌SGC7901细胞增殖、凋亡的体外实验. 中国细胞生物学学报, 2018, 40(1):71-81.
19.LEE C Y, SIT W H, FAN S T, et al. The cell cycle effects ofdocosahexaenoie acid human metastatic hepato cell ularcarcinoma proliferation［J］. Int J Oncol, 2010, 36(4):991-998.
20.GAO K,LIANG Q, ZHAO Z H, et al. Synergistic anticancer properties of docosahexaenoic acid and 5-fluorouracil through interference with energy metabolism and cell cycle arrest in human gastric cancer cell line AGS cells［J］. World J Gastroenterol, 2016, 22(10):2971-2980.
21.DRAPER E, REYNOLDS C M, CANAVAN M,et al.Omega-3 fatty acids attenuate dendritic cell function via NF-κB independent of PPARγ［J］. J Nutr Biochem, 2011, 22(8):784-790.
22.MANDAL C C, GHOST-CHOUDHURY T, YONEDA T, et al.Fish oil prevents breast cancer cell metastasis to bone［J］. Biochem Biophys Res Commun, 2010, 402(4):602-607.
23.LI W, CHEN Y Q, SHEN Y B,et al. HIF-1alpha knockdown by miRNA decreases survivin expression and inhibits A549 cell growth in vitro and in vivo［J］. Int J Mol Med, 2013, 32(2):271-280.
24.SAHN K, KHAN Z, KHAN G J, et al.Structural functional and therapeutic biology of surviving ［J］. Cancer Let, 2006, 244(2):164-171.
25.张徐宁, 戴翠萍, 夏前正, 等. 二十二碳六烯酸联合顺铂对人食管癌耐药细胞周期及凋亡的影响［J］. 现代预防医学, 2014, 41(14):2591-2594.