Clinical study on the changes of intestinal microflora and the end time of radiotherapy for cervical cancer

Sheng Xiang; Zhu Ruijuan; Li Suyi

doi:10.16689/j.cnki.cn11-9349/r.2020.03.014

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Electronic Journal of Metabolism and Nutrition of Cancer ›› 2020, Vol. 7 ›› Issue (3) : 321-326. DOI: 10.16689/j.cnki.cn11-9349/r.2020.03.014

Clinical study on the changes of intestinal microflora and the end time of radiotherapy for cervical cancer

Sheng Xiang1，2, Zhu Ruijuan 3, Li Suyi2

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Abstract

Objective To investigate the effect of the end time of radiotherapy on intestinal microflora in cervical cancer patients, providing experimental evidence for clinical prevention and treatment of intestinal infections. Methods Based on the end time of radiotherapy, patients were divided into short-term group within 3months, and patients were divided into long-term groups with 3months or more of the end time of radiotherapy. For sequencing and analysis of 16S rRNA gene v4 sequences of intestinal microflora, we chose Illumina MiSeq high-throughput sequencing platform. In addition, bioinformatics analysis of the results was processed by using a collection of tools such as QIIME 2, and to predict the functional metabolism of intestinal microbiota by PICRUSt. Results There was no significant difference in intestinal microbial richness and diversity between the two groups (P>0.05)；In the structure of intestinal microbial community, there was a significant difference between the two groups (P<0.05)；In terms of intestinal microorganisms composition, 5 bacterial genera with significant differences were selected from the two groups, namely f_Barnesielaceae_g_uncl, g_AlistiPes, g_Bacteroides, g_Blautia, and g_Desulfovibrio. In terms of functional metabolism, it was found that the metabolism type of intestinal microbiota in patients in the Long-term group appeared to the up-regulation of colitis-related metabolic pathways. However, in the short-term group, the metabolic types of the intestinal microbiota were mainly normal metabolic pathways. Conclusion Through the analysis of intestinal microflora gene sequencing and microecological study in two groups of cervical cancer patients, it was found that the end time of radiotherapy had a certain effect on the diversity and metabolic function of intestinal microflora. For patients whose intestinal microflora was completed more than 3 months ago, 5 bacterial genera and 1 abnormal metabolic pathway were found in their intestinal microflora, while no significant changes were seen in STG. This is for clinical prevention and treatment of radiotherapy-related intestinal flora imbalance provide a microbiological basis.

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Sheng Xiang, Zhu Ruijuan, Li Suyi. Clinical study on the changes of intestinal microflora and the end time of radiotherapy for cervical cancer[J]. Electronic Journal of Metabolism and Nutrition of Cancer. 2020, 7(3): 321-326 https://doi.org/10.16689/j.cnki.cn11-9349/r.2020.03.014

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