Abstract: Objective To study the effects of G. lucidum spore oil oral emulsion an enhancing the function of immune system.Methods H22 Tumor bearing mice as the research object were divided into six groups: negative control group (corn oil), positive control group (Kanglaite Soft Capsules), G. lucidum spore oil low dose group, G. lucidum spore oil high dose group, G. lucidum spore oil oral emulsion low dose group, G. lucidum spore oil oral emulsion high dose group. Observe the effect of G. lucidum spore oil oral emulsion on the function of humoral immunity, phagocytic function of reticuloendothelial system, delayed type allergic reaction and the effect of inhibiting tumor and biological regulation factors in H22 tumor bearing mice. Results G. lucidum spore oil oral emulsion 1.2g/kg could increase the serum HC50 value of H22 tumor bearing mice, could significantly enhance the clearance index of H22 tumor bearing mice, could improve the liver coefficient, spleen coefficient and phagocytic coefficient of H22 tumor bearing mice, was positive on DNFB induced delayed hypersensitivity reaction and could significantly increase the content of serum IL-1, TNF-alpha, IFN-gamma of H22 tumor bearing mice. Conclusions G. lucidum spore oil oral emulsion can enhance the immune function and improve the levels of biological regulatory factors IL-1, TNF-alpha, IFN-gamma of H22 tumor bearing mice.