Objective To investigate the anti-tumor effect of oral administration of ganoderma lucidum spores oil emulsion and its synergism and attenuation. Methods In this study, eighty mice were divided into 8 groups: control group, CP, CP+ ganoderma lucidum spore oil oral emulsion, low dose and high dose; ganoderma lucidum spore oil oral emulsion, low dose and high dose; Kanglaite soft capsule; CP+ Kanglaite soft capsule, with 10 rats in each group. To creating tumor bearing model, H22 cell line was inoculated into the back of the axilla of the mice. Giving administration in the treatment of ganoderma lucidum spores oil oral emulsion combined with chemotherapy drugs for 21 days, and observed whether tumor growth was inhibited. Results For oral administration of ganoderma lucidum spores oil emulsion combined with conventional chemotherapy drugs CP, there is a trend to enhance the effect of chemotherapy drugs to inhibit tumor growth. It can also improve the side effect of chemotherapy drugs on WBC, RBC, HGB. WBC (P<0.05) which was significantly increased in the high dose group of CP+ ganoderma lucidum spore oil emulsion. Ganoderma lucidum spores oil emulsion can reduce the damage of chemotherapy drugs on liver function. To improve the effect of chemotherapy drugs on the spleen index, thymus coefficient, the number of bone marrow cells, the number of cells in the reticulated red blood cells, and the reduction of the splenic nodules. Conclusions Ganoderma lucidum spores oil oral emulsion combined with chemotherapy drug CP, with certain tumor suppressor and attenuated synergistic effect and its mechanism may be related to enhance immunity, promote hematopoietic function of bone marrow.