Objective To explore the nutritional effect of short peptide enteral nutrition on intestinal mucositis rats. Methods42 model of intestinal mucosa was established by methotrexate injection. SD rats were randomly divided into blank control group (n=14) and control group (n=14). Successful models were randomly divided into 3 groups: normal diet group, protein enteral nutrition group, short peptide enteral nutrition preparation group. Rats were given daily diets of 38kcal/100g. The serum levels of total protein, albumin, proprotein, transferrin and retinol were measured in the tail of rats at day 0, 3, 9 and 15 days after feeding. Protein and diamine magnesium oxide, and the rats were sacrificed 15 days after feeding, and pathological examination was performed to evaluate the intestinal injury. ResultsThe model of intestinal mucosal injury was established by methotrexate injection. The body weight and feeding rate of the short-peptide-type were better than those of the normal diet group and the integrated protein group (P<0.05). The rats were sacrificed at day 15, and the serum total protein, albumin, proprotein and transferrin were measured (P<0.05), and the diamine oxidase was decreased (P<0.05) compared with normal feeding and integrated protein group, while HE staining and short peptide. The damage of the internal nutrition group was lighter than that of the other two groups. ConclusionsPeptide preparation for intestinal mucosal injury is not only beneficial to the improvement of nutritional status in rats, but also contributes to the recovery of injury.