Objective Exploring the correlation between the expression and prognosis of monounsaturated fatty acid related enzyme CBR1 polyunsaturated fatty acid related enzymes ALOX15 and FADS1 in epithelial cancer tissues. Method The expression levels of CBR1 ALOX15 and FADS1 were analyzed by immunohistochemistry and Western blot analysis from 65 epithelial ovarian cancer samples collected from the Department of Gynecology Affiliated Cancer Hospital of Harbin Medical University from April 2020 to December 2023. Analyze the expression of these three unsaturated fatty acid-related enzymes tumor cell proliferation correlation with overall patient survival and immune cell infiltration through GEPIA2 database Kaplan-Meier survival curve and TIMER2. 0 database. Result Study found that CBR1 was correlated with serum CA125 levels ALOX15 was correlated with epithelial ovarian cancer staging and P53 expression and FADS1 was correlated with differentiation degree all P<0. 05 . GEPIA2 database showed that FADS1 was statistically significant with tumor stage and CBR1 and FADS1 were positively correlated with tumor cell proliferation. Kaplan-Meier survival analysis showed that high expression of FADS1 predicted shorter survival while high expression of ALOX15 predicted longer survival. Western blot analysis of the expressions of three UFA-related enzymes in EOC tumor tissues and adjacent tissues showed that the expressions of CBR1 and ALOX15 were consistent with GEPIA2 database while the expressions of FADS1 were opposite. The expression of these three unsaturated fatty acid - related enzymes was correlated with immune cell infiltration macrophages and CD8 + T cells . Conclusion This study validated the correlation between the expression of CBR1 ALOX15 and FADS1 in epithelial ovarian cancer and the clinical prognosis of patients However the relationship between monounsaturated fatty acids or polyunsaturated fatty acids and tumor occurrence development and prognosis still requires a large amount of experimental data for verification.