摘要：目的 探讨Notch3对人肝癌细胞系HCC-LM3细胞凋亡、细胞克隆形成能力、细胞迁移能力的影响。方法 qRTPCR检测Notch3在肝癌细胞系中的表达，运用小RNA干扰技术下调Notch3在HCC-LM3细胞系的表达，用Annexin VFITC/PI双标记流式细胞术检测HCC-LM3细胞凋亡，用固体细胞培养技术和Transwell实验分别检测细胞克隆形成能力及细胞转移能力。结果 干扰Notch3表达可以促进HCC-LM3细胞凋亡，抑制HCC-LM3细胞克隆形成及细胞转移能力。结论 肿瘤细胞Notch3高表达与肿瘤恶性转化有关。

Abstract: Objective To explore the effects of Notch3 on apoptosis, cell clone formation and migration ability in human hepatocellular carcinoma cell line HCC-LM3. Methods Notch3 expression was detected by qRT-PCR in liver cancer cell line. Expression of Notch3 was downregulated by using small RNA interference technology in cell line HCC-LM3 and apoptosis was detected by Annexin V-FITC/PI double marking flow cytometry, while clone formation assay and transwell experiment were performed to detect cell clone forming and cell migration respectively in HCC-LM3. Results Interference of Notch3 expression in HCC-LM3 can promote HCC-LM3 cell apoptosis, inhibit cell clone formation and cell migration. Conclusions Over-expression of Notch3 may be related to tumor malignant transformation .