目的 探索清热解毒中药复方JC724治疗肿瘤代谢调节机制。方法 以前期实验已经证实具有明确抑制结直肠癌 生长的清热解毒复方JC724为研究对象，采用高通量技术检测JC724作用于结直肠癌细胞后的RNA表达谱变化，进而运用数据挖掘技术分析JC724治疗结直肠癌可能涉及的靶标基因、信号传导通路和蛋白互作网络。结果JC724作用于结直肠癌细胞后，有25个基因表达发生显著性差异，主要是与细胞紧密连接有关的claudin-4基因上调和与线粒体代谢密切相关的多种基因下调，如MT-CO1、MT-CO2、MT-ND4L、MT-ND1和MT-ATP8P1基因；JC724复方中的君药白花蛇舌草作用结直肠癌细胞后，324个基因表达发生显著性差异，其中上调基因95个、下调基因229个，前15位上调基因和前15位下调基因中与线粒体代谢相关的有MT-CO1、MT-CO2、MT-ND1、MT-ND2、MT-ND4L、MT-ATP8P1、BCL2L1和BBC3等8种基因；JC724与白花蛇舌草比较，检测126个基因表达发生显著性差异，其中上调基因47个、下调基因79个。结论 中药复方治疗肿瘤表现出复杂系统的复杂性、中药复方的整体性、非加和性和多靶性等特征，清热解毒中药JC724治疗肿瘤的主要分子机制可能与其调节线粒体代谢有密切关系。

Objective To clarify the mechanism of cancer treatment by detoxicating herbs JC724. Methods Colorectal cancer cell eukaryotic RNA expression change was analyzed usinghigh-throughput technique after HT-27 cell treated 1h by JC724, a kind of detoxicating herbs, which had a definiteinhibit ted effect to colorectal cancer in our previous studies. Results The effect of JC724 on colorectal cancer cells showed significant difference in 25 gene expression, which were claudin-4 genes and other mitochondrial metabolism genes, such as MT-CO1 and MT-CO2 gene, MT-ND4L, MT-ND1 and MT-ATP8P1 genes. There was a significant difference in the expression of 324 gene after HT-29 cell treated 1h by Spreading hedyotis herb, which was the “jun drug” in JC724, including 95 up-regulated genes and 229 down-regulated genes. The top up-regulated genes and top down-regulated genes associated with mitochondrial metabolism were MT-CO1, MT-CO2, MT-ND1, MT-ND2, MT-ND4L, MT-ATP8P1, BCL2L1and BBC3. Compared with the Spreading hedyotis herb group, there was detected significant differences in the expression of 126 genes in the JC724 group, including 47 up-regulated genes and 79 down-regulated genes. ConclusionsIt showed the characteristics of complex system, the integrity, non-additivity and multi-targetting in the treatment of tumor for Chinese medicine, and the main molecular mechanism is closely related to regulate mitochondrial metabolism in the treatment of cancer by detoxicating herbs JC724.