目的 三结构域蛋白 44(TRIM44)在喉鳞状细胞癌(LSCC)中的表达水平及其与预后的相关研究。 方法 选取 2018 年 10 月至 2019 年 10 月自贡市第一人民医院收治的 98 例 LSCC 患者,术中分别取其 LSCC 组织和癌旁组织,采用 qRT-PCR 法检测 TRIM44 mRNA 在 LSCC 组织、癌旁组织中的表达水平;根据 LSCC 组织 TRIM44 mRNA 表达量的平均值分为 TRIM44 低 表达组和 TRIM44 高表达组;分析 TRIM44 mRNA 表达水平与 LSCC 患者生存状况的关系;分析影响 LSCC 患者预后不良的危 险因素。 结果 与癌旁组织相比,LSCC 组织 TRIM44 阳性表达率显著升高(P<0. 05)。 与癌旁组织相比,LSCC 组织 TRIM44 mRNA 的表达水平显著升高(P<0. 05);TRIM44 mRNA 表达水平与淋巴结转移、分化程度、远处转移有关(P<0. 05)。 分析显 示,TRIM44 高表达组患者 3 年生存率低于 TRIM44 低表达组(P<0. 05);生存患者与死亡患者在肿瘤分化程度(低分化)、淋巴 结转移及远处转移比例方面存在显著差异。 (P<0.05)。 淋巴结转移(HR = 3.332)、TRIM44 mRNA 高表达(HR = 5. 365)是影响 LSCC 患者预后不良的危险因素(P<0.05)。 结论 TRIM44 mRNA 在 LSCC 组织中呈高表达,与 LSCC 患者临床特征及生存情况有关,有望成为潜在的 LSCC 预后生物标志物。

Objective To explore the expression of tripartite motif-containing protein 44 TRIM44 in laryngeal squamous cell carcinoma LSCC and its correlation with prognosis. Method A total of 98 patients with LSCC admitted to Zigong First People's Hospital from October 2018 to October 2019 were selected and their LSCC tissues and paracancer tissues were respectively taken during operation. The expression level of TRIM44 mRNA in LSCC tissues and adjacent tissues was detected by qRT-PCR. According to the mean value of TRIM44 mRNA expression in LSCC tissues they were divided into low TRIM44 expression group and high TRIM44 expression group. The relationship between TRIM44 mRNA expression level and survival status of LSCC patients was analyzed. To analyze the risk factors affecting the poor prognosis of LSCC patients. Result The positive expression rate of TRIM44 in LSCC tissues was significantly higher than that in paracancer group P < 0. 05 . The expression level of TRIM44 mRNA in LSCC tissues was significantly increased compared with that in adjacent tissues P<0. 05 . TRIM44 mRNA expression level was correlated with lymph node metastasis differentiation degree and distant metastasis P < 0. 05 . Analysis showed that the 3-year survival rate of patients with high expression of TRIM44mRNA was lower than that of patients with low expression of TRIM44 P<0. 05 . There were significant differences in the proportion of poorly differentiated lymph node metastasis and distant metastasis between surviving patients and deceased patients P < 0. 05 . Lymph node metastasis HR=3. 332 and high expression of TRIM44 mRNA HR= 5. 365 were risk factors for poor prognosis in LSCC patients P<0. 05 . Conclusion TRIM44 mRNA is highly expressed in LSCC tissues which is related to the clinical characteristics and survival of patients with LSCC and is expected to be a potential prognostic biomarker for LSCC.