目的  肾癌是由多种因素共同作用而导致，本文研究肾癌组织和肾癌细胞中 miR-205 的表达量，阐明肾癌患者临床 病理特征及预后与miR-205 表达量的关系和可能的作用机制。方法  2010 年2月至2014 年2月在湖北土家苗族自治州中心医 院泌尿外科选取人肾癌组织和癌旁正常组织样本60 例，采用RT-PCR 检测miR-205 的表达量，并探究患者的临床病理特征及 预后情况与其之间的关系；采用 miR-205 转染肾癌细胞株 ACHN，通过 MTT 检测过表达 miR-205 对肾癌细胞生长的影响；通 过Transwell 实验研究miR-205 对结肠癌细胞迁移的影响；分别采用免疫组化和Western blot 方法探究肾癌组织上皮间质转化 情况。结果  与癌旁组织相比肾癌组织中miR-205 处于低表达（P＜0.05）；miR-205 的表达量与肿瘤的浸润和复发情况密切 相关（P＜0.05），而与肿瘤的病理分期和临床分期无关（P＞0.05）。miR-205 过表达后发现肾癌细胞的生长受到显著的抑 制（P＜0.05），迁移效率显著降低（P＜0.05）。肾癌细胞存在上皮间质转化现象，miR-205 可能是通过抑制上皮间质转化 来抑制细胞的增殖和迁移。结论  miR-205 在肾癌组织中低表达，其参与肾癌进展，miR-205 的表达情况可为肾癌治疗提供 思路。

 Abstract: Objective Renal cell carcinoma is induced by a variety of factors. The expression of miR-205 in renal cell carcinoma and renal cell carcinoma was studied. The relationship between the clinicopathological characteristics and prognosis of patients with renal cell carcinoma and the expression of miR-205, and the mechanism of miR-205 were elucidated. Methods From February 2010 to February 2014, 60 cases of renal carcinoma tissue and normal tissue samples were selected from the Department of Urology at our hospital. Expression level of miR-205 was detected by RT-PCR analysis. We observed the function of miR-205 on biological behavior of ACHN cells in vitro, the ACHN cell was transfected with miR-205 to up-regulate miR-205 expression. MTT was used for evaluating cell proliferation in miR-205 up-expression. Transwell assays were employed for investigating the cell invasion and migration ability inmiR-205up-expression. Immunohistochemical was used to investigate the Epithelial mesenchymal transformation in ACHN cell. Results The expression of miR-205 was detected in renal carcinoma tissues and normal renal tissues, but the renal carcinoma tissues showed a significantly lower expression. The expression of miR-205 was correlated with infiltration and relapse of renal carcinoma (P<0.05), but showed no correlation with pathological stage or clinical stage of renal carcinoma (P<0.05). After mir-205 overexpression, it was found that the growth of renal cancer cells was significantly inhibited (P<0.05), and the migration efficiency was significantly reduced (P<0.05). There is epithelial mesenchymal transformation in renal carcinoma cells, and mir205 may inhibit cell proliferation and migration by inhibiting epithelial mesenchymal transformation. Conclusions The decreased expression of miR-205 were correlated with the progress of renal carcinoma. This result may provide new ideas for studying the molecular mechanism of renal carcinoma and develop a new therapeutic strategy for renal carcinoma in the future.