目的  研究人参皂苷、白藜芦醇、灵芝多糖及冬虫夏草四种植物化学物单药其及复方的体外抗癌活性，探讨四种 植物化学物联合使用是否具有协同抗癌作用。方法  体外培养A549、MCF-7、SW480三株癌细胞，CCK8法检测人参皂苷、 白藜芦醇、灵芝多糖、冬虫夏草单药及其复方对三株癌细胞生长抑制率的影响；流式细胞仪检测分析植物化学物GRGC 复 方阻滞癌细胞周期及诱导癌细胞凋亡的作用；透射电镜观察植物化学物GRGC复方诱导A549肺癌细胞凋亡的形态学变化。 结果  人参皂苷、白藜芦醇、灵芝多糖及冬虫夏草对A549、MCF-7、SW480 三株癌细胞均具有显著的抑制杀伤作用，并随 药物浓度的增加其杀伤活性更强，呈明显剂量效应关系。人参皂苷、白藜芦醇、灵芝多糖、冬虫夏草配制成复方联合用药时， 其杀伤效应较单药显著增强，有明显的协同抗癌效应。流式细胞周期分析显示植物化学物GRGC 复方能将A549、MCF7、SW480 三株癌细胞周期阻滞于G0/G1 期。流式细胞凋亡分析GRGC 复方诱导A549、MCF-7、SW480 三株癌细胞凋亡 呈剂量依赖性。在透射电镜下可观察到GRGC 复方能诱导A549 癌细胞细胞膜破损、核被膜消失、胞核逐渐浓缩聚集成新 月状或形成凋亡小体。结论  人参皂苷、白藜芦醇、灵芝多糖、冬虫夏草 GRGC 复方对体外培养的 A549、MCF-7、SW480 三株癌细胞的抑制杀伤效率比单药显著增强，具有明显的协同抗癌效应，能阻滞A549、MCF-7 和 SW480 癌细胞周期于 G0/G1 期，阻滞癌细胞增殖，并能有效诱导 A549、MCF-7、SW480 癌细胞凋亡，抑制癌细胞的生长 , 为肿瘤的综合治疗打 下坚实的基础。

 Objective  The study aims in investigating anti-tumor activity of ginsenoside, resveratrol, ganoderma polysaccharides and cordyceps sinensis in human cancer cell lines, and whether the combination of all four phytochemicals (GRGC) exerts synergistic anti-tumor effect. Methods  Human cancer cell lines (A549, MCF-7 and SW480) were treated with single formulation of Ginsenoside, Resveratrol, Ganoderma polysaccharides or Cordyceps sinensis, or the combined GRGC. All treated cells were analyzed for proliferation by CCK8 assay. Analysis of cell cycle arrest and apoptosis were conducted in cells treated with combined GRGC by flowcytometry. Furthermore, the ultrastraccture of A549 cells treated with GRGC was investigated by transmission electron microscope. Results  Ginsenoside, resveratrol, ganoderma polysaccharides and cordyceps sinensis exerted dose-dependent inhibition of proliferation in A549, MCF-7 and SW480 cells, while the mixed GRGC demonstrated significant synergistic effect in antitumor activity. After treatment with the GRGC, A549, MCF-7 and SW480 cells were arrested in the G0/G1 phase. Flowcytometry analysis revealed that the mixture of GRGC could induce apoptosis in A549, MCF-7 and SW480 cells in a dose-dependent manner. Electronphotomicrograph of GRGC treated A549 tumor cells showed that the cell membrane and nuclear envelope were broken, the nucleus concentrated and aggregated along the border, indicating massive or crescent like apoptosis. Conclusions  The mixture of the four phytochemicals GRGC exhibits significant synergy in inhibitory activities against tumor cells (A549、MCF-7、SW480), induces cell cycle arrest in the G0/G1 phase to inhibit tumor cell proliferation and to induce apoptosis of tumor cells (A549、MCF7、SW480). The combination of all four phytochemicals (GRGC) have obvious synergistic anticancer effect, which provides a solid foundation for the comprehensive treatment of cancer.