目的 探讨血管生长因子（VEGF）、转化生长因子β1、程序性死亡因子配体1（PD-L1）在不同卵巢肿瘤组织中的表达情况和三者分别与上皮性卵巢癌临床病理特征之间的关系。方法 采用免疫组化法分别检测46例上皮性卵巢癌、20例良性卵巢囊腺瘤及20例正常卵巢中VEGF、TGF-β1及PD-L1的表达情况，并分析其表达与不同临床病理特征之间的相关性。结果 免疫组化显示VEGF在上皮性卵巢癌组织、良性卵巢囊腺瘤组织及正常卵巢组织中阳性表达率依次为65.2%（30/46）、25.0%（5/20）、15.0%（3/20）；TGF-β1在上皮性卵巢癌组织、良性卵巢囊腺瘤组织及正常卵巢组织中阳性表达率依次为82.6%（38/46）、30.0%（6/20）、20.0%（4/20）；PD-L1在上皮性卵巢癌组织、良性卵巢囊腺瘤组织及正常卵巢组织中阳性表达率依次为89.1%（41/46）、30.0%（6/20）、25.0%（5/20），VEGF、TGF-β1及PD-L1在上皮性卵巢癌组织中的阳性率均高于良性卵巢囊腺瘤组织和正常卵巢组织，差异有统计学意义（P<0.01）。VEGF、TGF-β1及PD-L1在上皮性卵巢癌组织中的表达均与国际妇产科协会（FIGO）分期及淋巴结转移有关（P<0.05），其中TGF-β1的表达还与CA125升高有关（P<0.05）。在上皮性卵巢癌组织中，VEGF水平与TGF-β1呈正相关（r=0.387，P<0.05）；VEGF与PD-L1呈正相关（r=0.332，P<0.05）；TGF-β1与PD-L1呈正相关（r=0.393，P<0.05）。结论 在上皮性卵巢癌组织中VEGF、TGF-β1及PD-L1表达显著增高，三者呈正相关，且均与FIGO分期及淋巴结转移相关，提示VEGF、TGF-β1和PD-L1三者之间可能通过某种机制相互作用，协同促进上皮性卵巢癌的进展，联合检测可能指导上皮性卵巢癌的早诊断早诊治。
Abstract
Objective To investigate the expressions of VEGF, TGF-β1 and PD-L1 proteins in epithelial ovarian cancer, and to explore the association of the expressions of VEGF, TGF-β1 and PD-L1 with clinicopathological features. Methods Immunohistochemistry was used to detect the expressions of VEGF, TGF-β1 and PD-L1 in 46 cases of epithelial ovarian cancer, 20 cases of benign ovarian cystadenoma and 20 cases of normal ovarian, and analyzing the association of the expressions of VEGF, TGF-β1 and PD-L1 with the clinicopathological features. Results Immunohistochemistry showed that the positive expression rates of VEGF in epithelial ovarian cancer, benign ovarian cystadenoma tissue and normal ovarian tissue were 65.2% (30/46), 25.0% (5/20) and 15.0% (3/20), respectively. The positive expression rates of TGF-β1 in epithelial ovarian cancer, benign ovarian cystadenoma tissue and normal ovarian tissue were 82.6% (38/46), 30.0% (6/20) and 20.0% (4/20), respectively. The positive expression rates of PD-L1 in epithelial ovarian cancer tissues, benign ovarian cystadenoma tissue and normal ovarian tissue were 89.1% (41/46), 30.0% (6/20) and 25.0% (5/20), respectively. The positive rates of VEGF, TGF-β1 and PD-L1 in epithelial ovarian cancer tissues were higher than those in benign and normal ovarian tissues, with statistically significant differences (P<0.01). The expression of VEGF, TGF-β1 and PD-L1 in epithelial ovarian cancer was associated with FIGO staging and lymph node metastasis (P<0.05), and TGF-β1 was also associated with CA125 (P<0.05). In epithelial ovarian cancer,VEGF level was positively correlated with TGF-β1 (r=0.387, P<0.05)；VEGF level was positively correlated with PD-L1 (r=0.332, P<0.05)；TGF-β1 was positively correlated with PD-L1 (r=0.393, P<0.05). Conclusions The expressions of VEGF, TGF-β1 and PD-L1 were significantly up-regulated in epithelial ovarian cancer tissues and the above factors showing a positive correlation. The correlation with FIGO staging and lymph node metastasis suggested that VEGF, TGF-β1 and PD-L1may interact with each other through some mechanism to promote the progress of epithelial ovarian cancer, and the combined detection may guide the early diagnosis and treatment of epithelial ovarian cancer.
关键词
卵巢肿瘤｜基因｜血管内皮生长因子｜转化生长因子&beta /
1｜程序性死亡因子配体1
Key words
Ovarian neoplasms｜Gene｜Vascular endothelial growth factor｜Transforming growth factor-β1｜Programmed cell death /
ligand 1
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