目的 标化天然植物中抗癌化合物 JC724 的提取方法，并观察 JC724 对结直肠癌的作用。方法 采用 SOP 程序
从药食同源食品和抗癌中药处方中提取 JC724，用 Phytomics QC 系统评价提取产物的稳定性和生物学效应，确定组方中原
料比例和提取条件参数，研究 JC724 对结直肠癌的体内外抑制作用。结果 使用 10 组不同条件的 JC724 提取方案，获得了
30 批次 JC724，Phytomics QC 系统评价其中一组获得的 3 批次 JC724 具有最稳定生物学活性，该组提取方法即为 JC724 标
准化提取方法。96 小时内 JC724（20mg/ml）、5-Fu（5mg/ml）、5-Fu+JC724（5-Fu：2.5mg/ml，JC724：10mg/ml）对 293
细胞的最高抑制率分别为 93.2%、82.1%、3.9%，JC724 对 293 细胞无抑制作用（P ＞ 0.05）。JC724、5-Fu、5-Fu+JC724
对三株结直肠癌细胞株 HCT-116、HT-29、SW-480 均有抑制作用。96 小时内 JC724、5-Fu、5-Fu+JC724 对 HCT-116 细胞
最高抑制率分别为 92.5%、97.0%、100%，组间无显著性差异（P ＞ 0.05）；对 HT-29 细胞最高抑制率分别为 84.2%、
91.7%、98.5%，组间有显著性差异（P ＜ 0.05）；对 SW-480 细胞最高抑制率分别为 84.3%、98.6%、100%，JC724 组与其
他两组有显著性差异（P ＜ 0.05）。JC724、5-Fu、JC724+5-Fu 治疗 9 天后对结直肠癌荷瘤小鼠的抑瘤率分别为 83.95%、
85.60%、92.07%，JC724+5-Fu 组与 JC724 组、5-Fu 组比较有非常显著性差异（P ＜ 0.01），对照组、JC724 组、5-Fu 组、
JC724+5-Fu 组小鼠体重依次是起始体重的 106%、104%、73%、86%，后三组之间有非常显著性差异（P ＜ 0.01）。结论
Phytomics QC 系统是质控植物活性成分提取的一种理想工具，JC724 对结直肠癌具有较好的抑制作用，与 5-Fu 联合应用具
有协同和减毒作用。
 

 Objective To standardize the extraction condition of JC724 from natural plants, and to test the effect of JC724 on
colorectal cancer. Methods Standard operating procedure (SOP) was adopted to extract JC724 from preset medicine homologous
food and traditional anti-tumor Chinese medicine. Phytomics QC system was used to test stability of the extraction methods and to
examine the biological effect of JC724. By standardization of the extraction conditions and methods of JC724, the effect of inhibition
on colorectal cancer in vivo and in vitro was studied and compared with 5-Fu. Results 30 batches of JC724 were acquired using SOP
through ten different extraction conditions and methods (3 batches per set). Phytomics QC system was used to test for JC724. The
condition and method for the sample set which had the most stable biological activities was confirmed as standard extraction method.
During 96-hour, the highest inhibition rate on 293 cell line among JC724(20mg/ml), 5-Fu (5mg/ml), 5-Fu combined with JC724 (5-Fu:
2.5mg/ml, JC724: 10mg/ml) were 93.2%、82.1%、3.9% respectively, no effects was found in JC724 to 293 cell (P>0.05). The 96-
hour highest inhibition rate on HCT-116 among JC724, 5-Fu and 5-Fu+JC724 were 92.5%, 97.0% and 100% respectively (P>0.05).
However, the 96-hour inhibition rate on HT-29 among JC724, 5-Fu and 5-Fu+JC724 were 84.2%, 91.7% and 98.5% respectively
(P<0.05), and similar results were found in SW-480 cell line, the 96-hour inhibition rate on HT-29 among JC724, 5-Fu and 5-Fu+JC724
were 84.3%, 98.6% and 100% respectively (P<0.05). In colorectal tumor bearing mice model, the tumor inhibition rate of JC724, 5-Fu
and JC724+5-Fu were 83.95%, 85.60%and 92.07% respectively. After 9 days treatment, the weight of the control, JC724, 5-Fu and
JC724+5-Fu treated mice group were 106%, 104%, 73% and 86% of its primary weight respectively (P<0.01 among JC724, 5-Fu and
JC724+5-Fu group). Conclusions Phytomics QC system was a feasible evaluation tool for standardization of JC724, served as an
inhibitor on colorectal cancer in vivo and in vitro, which had synergistic effect with 5-Fu and might reduce the toxicity of 5-Fu.