目的 活性乳清蛋白（active whey protein）是采用低温专利萃取技术获得的牛乳中以乳清蛋白为主的产品，蛋白 含量高达90%以上，具有改善营养状况和增强免疫力等多种生物学作用，是临床营养治疗中提供机体营养的重要组分之一。 本研究构建胰腺癌荷瘤鼠模型，探讨活性乳清蛋白在胰腺癌营养治疗中的疗效。方法 构建胰腺癌panc-1 细胞裸鼠皮下移 植瘤模型，根据不同的营养干预方式随机分成 3 组，每组 8 只，分别为标准饲料喂养组（SD 组）、添加黄豆蛋白粉饲养组 （Soy 组）、添加活性乳清蛋白饲养组（ABD 组），观察不同喂养前后小鼠生活状态、体质量、肿瘤体积的变化情况，比 较小鼠的平均生存周期，同时检测血液及瘤组织谷胱甘肽含量。结果 实验过程中，三组小鼠均出现了体重下降，ABD 组 小鼠治疗8 周后，体重为（24.35±1.89）g，显著高于Soy 组（19.24±2.40）g 和 SD 组（20.04±2.41）g（P ＜ 0.05）；三组 移植瘤体积和瘤组织质量无显著差异；至实验终点，ABD 组平均生存周期为（62.13±2.47）天，与Soy 组（51.63±10.54） 天和SD组（56.00±5.29）天比较，差异均有统计学意义（P＜0.05），Soy组和SD组生存期差别无统计学意义（P＞0.05）； ABD 组血液 GSH 含量明显高于 Soy 组和 SD 组（P ＜ 0.05），而 ABD 组瘤组织 GSH 含量低于 Soy 组和 SD 组，但差别无 统计学意义（P ＞ 0.05）。结论  活性乳清蛋白可预防荷瘤鼠的体重下降，明显延长生存期，可能与其防治肿瘤恶液质的发 生有关。

Objective Active whey protein preserved the active ingredients in cow milk processed with patented low temperature extraction technology. Protein contents up to 90%. In addition, it has many biological functions such as improving the nutrition status and strengthening immunity. It’s one of the important components in clinical nutritional therapy. This research is to study the effect potential mechanism and potentiof active whey protein on pancreatic carcinoma established in vivo model. Methods Xenotransplanted pancreatic carcinoma was established by panc-1 cell line, then divided into 3 groups, 8 mice of each randomly according to the different way of nutritional intervention: standard diet group (SD group), standard diet with soy group (Soy group), standard diet with active whey protein group (ABD group). During the experiment, animals' body condition was observed, weight of nude mice, tumor size and weight were recorded. And, compared the average survival time of mice. Meanwhile, we detected the glutathione levels in the blood and carcinoma. Results Weight loss of all mice in the three groups was observed. Weight of mice in ABD group (24.35±1.89)g was significantly higher than SD group (20.04±2.41)g 8 weeks after treatment (P<0.05). Volumn and weight of mice transplanted tumor in the three groups was no statistical difference. At the end point, compared with the SD group and Soy group [(56.00±5.29)d and (51.63±10.54)d, respectively], the average survival time of mice in ABD group(62.13 ± 2.47)d was significantly higher (P<0.05). There was no statistical difference between SD group and Soy group (P>0.05). The GSH level in the blood of ABD group was significantly higher than SD group and Soy group (P<0.05) when the level in the tumor of ABD group was lower but there was no statistical difference. Conclusions Active whey protein can prevent the weight loss in transplanted pancreatic mice, prolong the survival time, and this may be associated with preventing and curing cancer cachexia.