为了探讨cyclin D1(CCNDl)A870G 基因多态性与保定地区结肠癌患者的年龄、性别、高胆固醇血症、家族史、 病理分型、病理分期的关系，我们从200 例保定地区结肠癌患者和200 例保定地区健康体检者的血中提取DNA，用聚合 酶链反应和限制性酶切片段长度多态体分析对提取的 DNA 进行多肽性分析。结果显示，病例组和对照组 AA\ AG\GG 基因 频率无明显差异。高胆固醇及重度吸烟的结肠癌患者GG 基因型频率明显高于其他基因型，有明显差异（P<0.05），重度 吸烟及高胆固醇血症在健康体检人群中GG 基因型无明显差异（P>0.05）。由此推断高胆固醇血症及吸烟可作为在cyclin D1(CCNDl)A870G 基因多态性中GG 基因型中结肠癌发生的危险因子，遗传因素的结肠癌患者AA 基因型频率明显高于其 他基因型，有明显差异（P<0.05）。由此推断遗传因素可作为在cyclin D1(CCNDl)A870G 基因多态性中AA 基因型中结肠 癌发生的危险因子，这将为进一步研究结肠癌分子生物学的发生机制提供依据。

To study the relationship between the cyclin D1(CCNDl)A870G gene polymorphism and age, sex, smoking, family history, pathological typing and tumor stage for the patients with colon cancer (in Baoding). Cyclin D1(CCNDl)A870G genotype wasdetermined by polymerase chainreaction (PCR) and restriction fragment length polymorphism analysis (RFLP) of DNA extracted from blood. The study included 200 colon cancer patients and 200 healthy control subjects. Genotype frequencies of patients and controls both conformed to the hardy equilibrium. The GG genotype significantly correlated with a history of heavy smoking and Hypercholesterolemia (P<0.05). We conclude that hypercholesterolemia and smoking may be a risk factor for colon cancer in the GG genotype of cyclin D1(CCNDl)A870G polymorphism, the frequency of AA genotype in patients with hereditary factors was significantly higher than that of other genotypes, (P<0.05). There was no significant difference in GG genotype between severe smokers and hypercholesterolemia patients (P>0.05). It can be concluded that genetic factors can be used as a risk factor for colon cancer in the AA genotype of cyclin D1(CCNDl)A870G polymorphism, which will provide a basis for further study on the molecular mechanism of colon cancer.