目的探讨卵巢癌腹水中3种不饱和脂肪酸差异代谢物的临床意义。方法本研究采用了高通量、高敏感性的代谢 组学研究方法，选取37例卵巢癌腹水（包括上清和沉淀细胞）与22例卵巢癌组织样本，按照取腹水时是否进行了化疗分为未化疗 组和铂类化疗组，将复发的腹水样本分为敏感组和耐药组，敏感与耐药两个对照组进行超高效液相色谱串联飞行时间质谱UHPLCQ- TOF / MS分析，结合人类代谢物数据库（HMDB）与多元统计学分析在两组中各检测出了46个差异代谢物。从脂肪酸代谢角 度筛选出3种参与不饱和脂肪酸（UFA）生物合成的差异代谢物，分别为花生酸、肾上腺酸和二高-γ-亚麻酸（DGLA）。结果通 过代谢物质谱峰强度变化以及对数据库中上下游相关酶的编码基因［酰基辅酶A硫酯酶（ACOT编码）、去饱和酶（FADS编码）等］ 与卵巢癌标志物的相关性分析，显示它们代谢水平的改变与患者血清CA125等标志物的表达水平呈正相关。结论卵巢癌腹水 中参与UFA代谢的花生酸、肾上腺酸和DGLA代谢水平的改变可对卵巢癌的预后产生一定的影响。

Objective To investigate the clinical significance of differential metabolites of three unsaturated fatty acids in ascites of ovarian cancer. Methods According to no chemotherapy and chemotherapy, chemosensitivity and chemotherapy resistance,37 cases of ovarian cancer ascites (including supernatant and precipitated cells) and 22 cases of ovarian cancer tissue samples were selected and divided into two groups for ultra-high performance liquid chromatography tandem time-of-flight mass spectrometry UHPLC-Q-TOF / MS analysis, combined with human metabolome database and multivariate statistical analysis, 46 differential metabolites were detected in each of the two groups. From the point of view of fatty acid metabolism, three differential metabolites involved in biosynthesis of unsaturated fatty acids (UFA) were screened.They are adrenic acid ,arachidic acid and dihomo-γ-linolenic acid(DGLA).Results The changes of metabolic peak intensity and the correlation between the coding genes of upstream and downstream related enzymes in the database [acyl-CoA thioesterase (ACOT code), desaturase (FADS code), etc.] showed that the changes of their metabolic levels were positively correlated with the expression levels of serum CA125 and other markers in patients. Conclusion Changes in the metabolic levels of arachidic acid,adrenic acid and DGLA in ascites of ovarian cancer may affect the prognosis of ovarian cancer to a certain extent.