目的研究富含脯氨酸蛋白11（PRR11）在肝细胞癌（HCC）组织中的表达及其对HCC细胞生物学行为的影响。方法 采用基因表达谱数据动态GEPIA分析PRR11在不同HCC组织和癌旁组织中表达并分析与患者预后的影响；构建PRR11沉默细 胞系，通过实验验证PRR11对HCC细胞生物学行为。结果GEPIA分析发现，HCC组织中PRR11的水平更高（P<0.05）；PRR11低 表达HCC患者生存时间明显更长（P<0.05）；sh‐PRR11组细胞在48 h、72 h和96 h的OD值分别为（0.32±0.05）、（0.43±0.06）、（1.25± 0.05），试验组OD值分别为（0.52±0.05）、（0.73±0.06）、（1.81±0.08），经重复性方差分析显示两组间不同时间点差异具有统计学意 义（P<0.05）；sh‐PRR11组侵袭细胞数和细胞迁移率分别为（36.15±2.06）%、（42.36±2.12）%，明显低于试验组的（83.25±6.35）%、（80.42± 3.64）%；sh‐PRR11组形成的肿瘤体积为（43.85±3.52）mm3，明显大于试验组的（[ 22.68±2.86）mm3，（P<0.05）]；sh‐PRR11组E‐cadherin 相对表达量为（1.63±0.52），明显高于试验组（1.03±0.61），sh‐PRR11组N‐cadherin相对表达量为（0.72±0.43），明显高于试验组（1.01± 0.54），差异均具有统计学意义（P<0.05）。结论PRR11在HCC中高表达，沉默PRR11可抑制HCC细胞，可能与PRR11对上皮间 质转化过程具有调控作用有关。

Objective To investigate the expression of proline protein 11 (PRR11) in hepatocellular carcinoma (HCC) tissues and its effect on the biological behavior of HCC cells. Methods Gene expression profile data dynamic GEPIA was used to analyze the expression of PRR11 in HCC tissues and paracancerous tissues and its influence on the prognosis of patients. PRR11 silencing cell lines were constructed to verify the biological behavior of PRR11 on HCC cells through experiments. Results GEPIA analysis showed that The level of PRR11 was higher in HCC tissues (P<0.05). The survival time of HCC patients with low expression of PRR11 was significantly higher (P<0.05). The OD values of SH⁃PRR11 group at 48 h, 72 h and 96 h were (0.32±0.05), (0.43±0.06) and (1.25±0.05), respectively, and those of NC group were (0.52±0.05), (0.73±0.06) and (1.81±0.08), respectively. Repeatability analysis of variance showed that the difference between the two groups at different time points was statistically significant (P<0.05). The number of invaded cells and cell migration rate in SH⁃PRR11 group were (36.15±2.06) % and (42.36±2.12) %, respectively, which were significantly lower than (83.25±6.35) % and (80.42±3.64) % in NC group. The tumor volume of SH⁃PRR11 group was (43.85±3.52) mm3, which was significantly higher than that of NC group (22.68± 2.86) mm3 (P<0.05). The relative expression level of E⁃cadherin in SH⁃PRR11 group was (1.63±0.52), which was significantly higher than that in NC group (1.03±0.61), and the relative expression level of N⁃cadherin in SH⁃PRR11 group was (0.72±0.43), which was significantly higher than that in NC group (1.01±0.54). The differences in the above indexes were statistically significant (P<0.05).Conclusion PRR11 is highly expressed in HCC, and silencing PRR11 can inhibit the malignant behavior of HCC cells. The mechanism may be related to the regulatory role of PRR11 in the process of epithelial mesenchymal transformation.