目的 评估LYRM4‐AS1在胶质瘤患者中的表达水平和临床意义，以及其相关机制。方法 从TCGA数据库收集胶 质瘤患者的RNA‐seq数据与临床资料，研究LYRM4‐AS1在肿瘤组织与正常组织中的表达情况，分析其与胶质瘤患者临床病理 特征的关系。通过Kaplan‐Meier生存曲线和Cox回归分析，评估LYRM4‐AS1的临床意义，对LYRM4‐AS1潜在的生物学机制进 行探讨。结果 相比于正常组织，LYRM4‐AS1在多种肿瘤组织中高表达，并且与胶质瘤患者的世界卫生组织（WHO）等级（P< 0.001)、IDH状态（P<0.001)、1p/19q编码缺失（P<0.001)、初始治疗结果（P<0.001)、表皮生长因子受体状态（P<0.001）和年龄（P< 0.001）具有相关性。预后分析发现，LYRM4‐AS1 高表达胶质瘤患者具有更短的总生存期(HR=3.99，95%CI=3.01~5.30，P< 0.001), 无进展间隔生存期（HR = 2.68，95%CI=2.13~3.38，P<0.001）和疾病特异性生存期(HR=4.00，95%CI=2.98~5.37，P<0.001） 和PFI (HR=2.68，95%CI=2.13~3.38，P<0.001)。多因素Cox回归分析发现，LYRM4‐AS1为预后独立危险因子。功能富集分析发 现，LYRM4‐AS1与炎症和免疫反应相关。结论 LYRM4‐AS1可能是潜在的诊疗及预后标志物，并且其可能通过炎症与免疫 反应在胶质瘤发生发展中发挥作用。

Objective To evaluate the expression level and clinical significance of LYRM4⁃AS1 in glioma patients, as well as its related mechanisms. Methods RNA⁃seq data and clinical data of glioma patients were obtained from the TCGA database. The expression of LYRM4⁃AS1 in tumor and normal tissues was studied, and then the relationship between LYRM4⁃AS1 and the clinicopathological characteristics of patients with glioma was analyzed. Kaplan⁃Meier survival curve and Cox regression analysis were used to evaluate the clinical significance of LYRM4⁃AS1. The potential biological mechanism of LYRM4⁃AS1 was discussed through functional enrichment analysis. Results The study found that compared with normal tissues, LYRM4⁃AS1 was highly expressed in a variety of tumor tissues, and was related to WHO grade (P<0.001), IDH status (P<0.001), and 1p/19q coding loss (P<0.001), primary therapy outcome (P<0.001), EGFR status (P<0.001) and age (P<0.001). Prognostic analysis found that glioma patients with LYRM4⁃AS1 overexpressing had worse OS (HR=3.99, 95%CI=3.01-5.30, P<0.001), DSS (HR=4.00, 95%CI=2.98-5.37, P<0.001) and PFI (HR=2.68, 95%CI=2.13-3.38, P<0.001). Multivariate Cox regression found that LYRM4⁃AS1 was an independent risk factor for prognosis. Finally, functional enrichment analysis found that LYRM4⁃AS1 was related to inflammation and immune response. Conclusion LYRM4⁃AS1 may be a potential diagnosis, treatment and prognostic marker, and it may play a role in the occurrence and development of glioma through inflammation and immune response.