目的 探讨核仁纺锤体相关蛋白 1(NuSAP1)、低密度脂蛋白受体(LDLR)与局部晚期乳腺癌患者腋窝淋巴结转移情 况的关系,并分析患者腋窝淋巴结转移≥10 枚的危险因素。 方法 选取 2018 年 1 月至 2022 年 4 月在江苏省连云港市第一人 民医院诊治的局部晚期乳腺癌患者 125 例为研究对象,均经手术病理确诊为乳腺癌且均发生腋窝淋巴结转移。 术中取癌组织 及癌旁组织,并检测 NuSAP1 mRNA、LDLR mRNA 表达情况。 对比不同腋窝淋巴结转移情况患者癌组织 NuSAP1 mRNA、LDLR mRNA 表达,分析 NuSAP1 mRNA、LDLR mRNA 表达与腋窝淋巴结转移情况的相关性。 采用二元 Logistic 回归模型分析腋窝淋 巴结转移≥10 枚的危险因素。 结果 癌组织 NuSAP1 mRNA、LDLR mRNA 表达高于癌旁组织(P<0. 05);随着患者腋窝淋巴结 转移数目增加,癌组织 NuSAP1 mRNA、LDLR mRNA 表达呈升高趋势(P<0. 05);癌组织 NuSAP1 mRNA、LDLR mRNA 表达与 腋窝淋巴结转移数目呈正相关(P<0. 05);校正临床分期、肿瘤最大直径后,癌组织 NuSAP1 mRNA、LDLR mRNA 高表达仍是乳 腺癌患者腋窝淋巴结转移≥10 枚的独立危险因素(P<0. 05),建立 Logistic 回归模型:logit(P)= -6. 004+1. 298×LDLR mRNA+ 1. 141×NuSAP1 mRNA。 结论 局部晚期乳腺癌患者癌组织 NuSAP1 mRNA、LDLR mRNA 呈高表达,表达水平与患者腋窝淋巴 结转移数目密切相关,且 NuSAP1 mRNA、LDLR mRNA 表达升高是患者腋窝淋巴结转移≥10 枚的独立危险因素。

Objective To investigate the relationship between nucleolar spindle - associated protein 1 NuSAP1 low - density lipoprotein receptor LDLR and axillary lymph node metastasis in patients with locally advanced breast cancer and analyze the risk factors for axillary lymph node metastasis ≥ 10. Method A total of 125 patients with locally advanced breast cancer who were diagnosed and treated in our hospital from January 2018 to April 2022 were selected as the research subjects. The tumor tissues and adjacent tissues were collected during operation and the expressions of NuSAP1 mRNA and LDLR mRNA were detected. The expressions of NuSAP1 mRNA and LDLR mRNA in cancer tissues of patients with different axillary lymph node metastasis were compared. The correlation between NuSAP1 mRNA and LDLR mRNA expression and axillary lymph node metastasis was analyzed. Binary Logistic regression model was used to analyze the risk factors of axillary lymph node metastasis ≥ 10. Result The expressions of NuSAP1 mRNA and LDLR mRNA in cancer tissues were higher than those in adjacent tissues P< 0. 05 . With the increase in the number of axillary lymph node metastases the expression of NuSAP1 mRNA and LDLR mRNA in cancer tissues increased P<0. 05 . The expression of NuSAP1 mRNA and LDLR mRNA in cancer tissue was positively correlated with the number of axillary lymph node metastasis P<0. 05 . After adjusting for clinical stage and maximum tumor diameter high expression of NuSAP1 mRNA and LDLR mRNA in cancer tissue were still independent risk factors for axillary lymph node metastasis ≥ 10 in breast cancer patients P<0. 05 . Logistic regression model was established logit P = -6. 004+ 1. 298×LDLR mRNA+ 1. 141×NuSAP1 mRNA. Conclusion NuSAP1 mRNA and LDLR mRNA expressed highly in cancer tissues of patients with locally advanced breast cancer and the expression levels are closely related to the number of axillary lymph node metastases in patients.