目的 探究结肠癌患者叉头框蛋白 P3(Foxp3)、促泛素结合衔接子(p62) 及核因子 κB(NF-κB)表达及与癌旁肿瘤 沉积形成相关性。 方法 选取河北北方学院附属第一医院普通外科 2019 年 1 月至 2022 年 1 月收治的结肠癌患者 80 例,根据 其是否合并癌旁肿瘤沉积分为对照组(36 例),沉积组(44 例)。 使用实时荧光定量 PCR 技术( qRT-PCR)检测患者 Foxp3、 p62、NF-κB mRNA 表达,使用免疫组化检测患者 Foxp3、p62、NF-κB 阳性细胞表达,使用蛋白质印迹法检测两组患者病灶组织 中 Foxp3、p62、NF-κB mRNA 相对蛋白表达量。 并使用多因素 Logistic 回归分析以上指标与患者癌旁肿瘤沉积形成相关性。 结果 比较两组基线资料,差异无统计学意义。 沉积组 Foxp3、p62、NF-κB mRNA 表达显著高于对照组,差异具有统计学意义 (P<0. 05);沉积组 Foxp3、p62、NF-κB 免疫组化阳性细胞占比表达显著高于对照组,差异具有统计学意义(P<0. 05);沉积组 Foxp3、p62、NF-κB 蛋白相对表达显著高于对照组,差异具有统计学意义(P<0. 05);多因素 Logistic 回归分析结果显示,Foxp3、 p62、NF-κB mRNA,Foxp3、p62、NF-κB 阳性细胞占比,Foxp3、p62、NF-κB 蛋白相对表达均与患者癌旁肿瘤沉积发生具有显著 相关性,其高表达均是患者发生癌旁肿瘤沉积的独立危险因素(P<0. 05)。 结论 合并癌旁肿瘤沉积结肠癌患者病灶 Foxp3、 p62、NF-κB mRNA,Foxp3、p62、NF-κB 阳性细胞占比及 Foxp3、p62、NF-κB 蛋白相对表达均升高,且其高水平表达是结直肠癌患者癌旁肿瘤沉积形成独立危险因素,在患者实际治疗中,应重视以上指标表达,有助于评估及预测患者病情进展。

Objective To explore the expression of Foxp3 p62 and NF-κB in patients with colon cancer and their correlation with the formation of adjacent tumor deposits. Method The research samples were collected from 80 colon cancer patients admitted to Department of General Surgery The First Affiliated Hospital of Hebei North University from January 2019 to January 2022 and were divided into control group 36 cases and deposition group 44 cases according to whether they had adjacent tumor deposits. The expression of Foxp3 p62 and NF-κB mRNA in patients was detected by qRT-PCR the expression of Foxp3 p62 and NF - κB positive cells in patients was detected by immunohistochemistry and Foxp3 p62 NF-κB in the lesion organizers of the two groups of patients were detected by western blotting experiment. NF-κB mRNA relative protein expression. Logistic multivariate regression was used to analyze the correlation between the above indicators and the formation of paracancerous tumor deposits in patients. Result The mRNA expressions of Foxp3 p62 and NF-κB in the deposition group were significantly higher than those in the control group and the difference was statistically significant P < 0. 05 . The difference was statistically significant in the control group P < 0. 05 the relative expressions of Foxp3 p62 and NF-κB proteins in the deposition group were significantly higher than those in the control group and the difference was statistically significant P<0. 05 Logistic multivariate regression analysis showed that Foxp3 p62 NF-κB mRNA the proportion of Foxp3 p62 NF-κB positive cells and the relative expression of Foxp3 p62 NF-κB protein were all significantly correlated with the occurrence of adjacent tumor deposits in patients. The independent risk factors in patients with adjacent tumor deposits P<0. 05 . Conclusion The Foxp3 p62 NF-κB mRNA the proportion of Foxp3 p62 NF-κB positive cells and the proportion of Foxp3 p62 and NF-κB positive cells in colon cancer patients with adjacent tumor deposits The relative expressions of p62 and NF-κB proteins were both increased and their high levels of expression were independent risk factors for the formation of paracancerous tumor deposits in patients with colorectal cancer. disease progression.